We're a little crazy, about science!

Posts tagged “research

Day 310: Review – Interfacing with alpha motor neurons in spinal cord injury patients

Figure 1 from the paper showing EMG recordings and the transformation using deconvolution to motor neuron spike trains
Figure 1 from the paper showing EMG recordings and the transformation using deconvolution to motor neuron spike trains

Spatiotemporal spinal maps of ipsilateral a-MNs. (A) Experimental set-up for ankle plantar flexion. (B) HD-EMG is decomposed into a-MN spike trains using a convolutive blind-source separation technique. (C) The spinal output to generate the neural drive to muscles is estimated from the a-MN spike trains.

The world is on fire, we’re protesting for a future, but today I have my review paper due so instead of writing about my frustrations I’m going to share my review. Today we’re looking at the effects of trans-spinal direct current stimulation (tsDCS) on alpha motor neurons and how we can determine that effect using electromyography. It’s actually a very cool paper, the work is well done, and it’s open source so you can read it if you’re interested.

(more…)


Day 296: Review – Spinal Rhythm Generation by Step-Induced Feedback and Transcutaneous Posterior Root Stimulation in Complete Spinal Cord–Injured Individuals

Black and white diagram showing the front and back of a human with the the placement of electrodes over the lower abdominal area and separate electrodes over T11-T12 of the spinal cord a third image to the right shows the side of the spinal column with spinal cord to depict the location of the back electrodes with relation to the spinal cord
Black and white diagram showing the front and back of a human with the the placement of electrodes over the lower abdominal area and  separate electrodes over T11-T12 of the spinal cord a third image to the right shows the side of the spinal column with spinal cord to depict the location of the back electrodes with relation to the spinal cord

Transcutaneous posterior root stimulation: Paravertebral and abdominal skin electrode placement (this is just a portion of figure 1 of this paper.

Normally I’m somewhat excited to post these, but with everything going on you’ll have to pardon my lack of joy. However, it’s been two weeks so I need to review another paper so I’m sharing it here as well as sending my PI a copy. The study is a few years old, but it’s open access so you can read it if you’re interested. Transcutaneous spinal cord stimulation (TSS) is one part of my research in case you couldn’t tell from all the spinal cord and TSS studies and posts. I find it interesting and it gives me hope that we can help a whole lot of people living with spinal cord injury. Anyway give it a read and get out there and protest for a better world.

(more…)


Day 282: Review – Transcutaneous spinal cord stimulation of the cervical cord modulates lumbar networks

Experimental Setup and tSCS electrode placement (A) H-reflexes were evoked via stimulation of the tibial nerve and recorded in the soleus muscle during a consistent background contraction of ≈5% peak muscle activity. The left leg was held static in an extended position, and stimulation to evoke the H-reflex was delivered with the left arm at 0°. (B) tSCS was delivered via two 2.5 cm round cathodic electrodes placed midline at C3-4 and C6-7 (cervical) or T11 and L1 (lumbar) spinous processes. Two 5 × 10 cm rectangular anodic electrodes were placed bilaterally over the iliac crests.
Experimental Setup and tSCS electrode placement (A) H-reflexes were evoked via stimulation of the tibial nerve and recorded in the soleus muscle during a consistent background contraction of ≈5% peak muscle activity. The left leg was held static in an extended position, and stimulation to evoke the H-reflex was delivered with the left arm at 0°. (B) tSCS was delivered via two 2.5 cm round cathodic electrodes placed midline at C3-4 and C6-7 (cervical) or T11 and L1 (lumbar) spinous processes. Two 5 × 10 cm rectangular anodic electrodes were placed bilaterally over the iliac crests.

Experimental Setup and tSCS electrode placement (A) H-reflexes were evoked via stimulation of the tibial nerve and recorded in the soleus muscle during a consistent background contraction of ≈5% peak muscle activity. The left leg was held static in an extended position, and stimulation to evoke the H-reflex was delivered with the left arm at 0°. (B) tSCS was delivered via two 2.5 cm round cathodic electrodes placed midline at C3-4 and C6-7 (cervical) or T11 and L1 (lumbar) spinous processes. Two 5 × 10 cm rectangular anodic electrodes were placed bilaterally over the iliac crests.

It’s that time again! My biweekly critical review paper is due for my PI. He gets a copy and so do all of you. This is a particularly interesting study that falls in line with a lot of research that I am doing, so it’s interesting to see how other groups are progressing. Overall I think this is a great study and while it is behind a paywall, I think I summed it up very well. The drawing they did (above) is awesome, especially for a scientific journal where we normally use simple line figures. Anyway, let’s get to it.

(more…)


Day 275: Summer Mentorship

planting a tree

It’s summer, so we’re taking a break from my DI…why?! series to talk about my favorite thing about working over the summer. We get to do mentoring! As you may recall, mentoring is my favorite part of the job. Over the summer we get students from high school, international students, as well as undergrad students from all over the place to visit our little lab.

(more…)


Day 268: Review – Magnetospinography visualizes electrophysiological activity in the cervical spinal cord

Reconstructed currents of MSG from the cervical spondylotic myelopathy subject. There are two different sets of plots, the furthest left shows the electrophysiological recording taken from the epidural space and the other set shows the reconstructed currents using MSG. They agree fairly well.
Reconstructed currents of MSG from the cervical spondylotic myelopathy subject. There are two different sets of plots, the furthest left shows the electrophysiological recording taken from the epidural space and the other set shows the reconstructed currents using MSG. They agree fairly well.

This is the results of the cervical spondylotic myelopathy subject. The left graphs are the ascending spinal cord evoked potentials (this was electrophysiological recordings taken from the epidural space) by stimulation of the lower thoracic cord showing conduction block at the C4/5 disc level. The right graphs are the reconstructed currents at the midline of the cervical spinal canal (red) and 2 cm lateral (blue). The leading component (the first waveform in red) attenuated and disappeared through C4–6, and the trailing component (the second waveform in red) disappeared at C5/6. The perpendicular inflow components greatly attenuated at C4/5 (the second waveform in blue).

Another two weeks, another critical review. This time I was more critical than review, unlike the last one where I was blown away at the possibilities. Why was I more critical with this one? Well in my opinion, the authors took a baby step when they should’ve taken a leap. All that aside, it is an interesting study and one I hope has several follow up experiments. This one is open access as well, so have a read for yourself if you’re interested.

(more…)


Day 266: Pandemic research pressure

Pandemic

Think of research like the post office, nor rain, nor sleet, nor snow, nor pandemic, we will be there working. My dumbass had to make the transition from design to human experimenting the year before a pandemic. I could be safely at home designing cool robots in solidworks, but no we need to collect human subject data and despite the pandemic, I’m feeling the pressure.

(more…)


Day 264: Summer Research

EEG cap

Pay no attention to my messy desk… instead look at the cool EEG cap I set up.

It’s supposed to be a busy summer for me. With the pandemic going on, I’m not sure how that will play out. Since we don’t know yet, let’s talk about how it was planned and then we can discuss how that changed thanks to the COVID-19 pandemic. This may be bad as far as my research timeline… maybe.

(more…)


Day 216: So I’m “essential” now…

pandemic

Fun fact, I love pandemic movies. Movies, not so much living through one. As humans we are selfish, greedy, prone to panic, and for being “evolved” we are so very, very stupid. Basically every dumb choice you see the characters in those movies make while screaming, “you idiot, that would never happen in real life!” Well, it’s happening and wow does it do a number on a persons mental health. But hey guess what? I’m essential, so let’s figure out what that means in a time where the world is practically on fire.

(more…)


Day 215: What now?!

look ahead

Social distancing is important, but maybe not this distant…

Well if you missed it, yesterday was our final post in the know your spinal cord series. I’m not crying, you’re crying! Now the question is, what does that mean for the blog? An excellent question, one I wish I would’ve asked myself!  Let’s take a look at some of the things that we will (probably) be discussing now that our designated topic has run its course.

(more…)


Day 136: A look forward

a look forward

The obligatory photo of a path that goes on into the distance (taken from the hike we went on during the neurotech confrence).

Yesterday we took a look back at the past decade. I’m not a fan of looking back, it’s not how I’ve survived this long, it’s not something I’m particularly comfortable with, and even though it wasn’t a bad thing to do, I would rather look forward and talk about the things that will be happening (hopefully) this year. Let’s ring in the new year with the to-do list for this year!

(more…)


Day 101: Holiday

Well today is thanksgiving, not a holiday I celebrate, but I’m happy to have the day off at least. I’ll be taking the day to get some work done and relax some. Deadlines are coming so I can’t take the whole day to myself, but at least it’s a low stress day.

Until next time, don’t stop learning!


Day 100: Graduating lab members

Day 100 - PhD defense offense

Image from: xkcd.com

Wow, time flies, we’re already on day 100. Still not half way done, but it certainly doesn’t feel like 100 days of writing. Today has been a busy day, which is why I’m writing so late. With the end of the term comes other projects that need to be addressed, namely my qualifying exam, final exams, and other lab members having their Ph.D defense. Yep, we have not one, not two, but three people defending their Ph.D this term.

(more…)


Day 99: Data request, a update

letters vs email

Sometimes emails can still be exciting!

I’ve got news! In my last post on the subject I said that I wasn’t expecting a response from the lab I was requesting data from until Monday, if at all. Well would you believe I got a response! It wasn’t a “here’s the data response,” but it is progress. Let me explain why.

(more…)


Day 98: Laptop troubles

Well it finally happened. My laptop looks pretty dead. Right now I have it in pieces while I try to figure out what went wrong with it. Thankfully I have a desktop computer that I use for all my heavy computing as a back up. Just one more expense I guess, I should be grateful that it wasn’t something more serious like the hard drive going out.

So short post, I know, but I have to get this fixed or find some sort of resolution since my laptop is an important part of how I get work done. I’ll have a longer post tomorrow, but for now I think that is it.

Until next time, don’t stop learning!


Day 97: Neural network models

Neural net

Today should be an interesting day for me. I plan to do a deep dive into the world of neural networks to better understand how they work and the best type to apply to my data. There are a few, quite a few in fact that I could use and really it depends on how I want to frame my problem, so let’s look at what I’ve got planned.

(more…)


Day 96: The art of requesting data

Day 96 - mice

I have some data. This shouldn’t be news, I’ve been talking about how I’m trying to train a model to predict an output using this data and all the woes that have come from the training process. Turns out I have a problem, well more like questions that need answers and for that I need help.

(more…)


Day 95: Housework day

Pokemon procrastination

 

Pokemon procrastination

If only it was super effective!!!!!!

So far things have been non-stop and today is no exception. I have a lot of housework to do and balancing that work with my school work (all while still trying to find time to relax some) is difficult. Nevertheless today is housework day (mostly).

(more…)


Day 94: Paper formatting

submtting a paper

Ugh!! After over six weeks my paper was rejected becuase it didn’t fall in line with what the editor wanted. This is frustrating because each journal has different formatting requirements and you cannot submit to multiple journals at once so we need to sit and wait.

(more…)


Day 93: QE date is set… again!

5 tacks on a single date on a calendar

5 tacks on a single date on a calendar

Exciting news!!!!! Well as you can tell from the title, my qualifying exam date is set. After some time emailing back and forth I finally just sent out a doodle poll to get everyone to vote on what time would work best for them. Last night, my last committee member added their availability to the poll and with that, I have a new time and date!

(more…)


Day 92: Missed meetings…

65874759

As you may be aware, I am planning an experiment! Cue upbeat music Unfortunately record scratch I’m nowhere near ready. It’s been a process for sure and we are (maybe?) back on track to get started soon. Today I had a meeting scheduled with my PI to discuss the details, but…

(more…)


Day 91: Did that really just happen?

windows10 update screen

Screw you auto update

For those of you following along, I’ve been trying to crack a predictive model using some novel (read: super secret PhD work) neural data. It’s been a journey and I’ve trained and tested about a dozen or so models, with varying success. Things have been flying pretty smooth the past few weeks as I try to create the best model I could possibly create. Unfortunately, technology had other plans for me.

(more…)


Day 90: Memory

memorization

I have a lot of memorization to do these days. Things that are important , steps to set things up, new biology, new techniques, new, new, new… well you get the point. Memorization is the bain of my existence. It’s not even a matter of not wanting to memorize something, it’s just old war wounds, no really.

(more…)


Day 89: The stress of life

stress

Today I get to play catch up. I’m running on not a lot of sleep, but there is so much to be done it isn’t funny. Ironically enough, it’s not all school either. I have life things catching up with me, car repairs that I need to do, house things, you know the usual day to day things that pile up despite our best efforts.

(more…)


Day 88: Experimental Headaches

EEG

EEG cap

I’ve talked about my impending deadlines a lot lately. I also mentioned that I had an experiment that I needed to do to meet a deadline, well it looks like we may or may not meet this goal. Let’s talk about the latest headaches.

(more…)