We're a little crazy, about science!

Posts tagged “experiment

The end of the experiment

Well today was a milestone for me, the end of an experiment I wasn’t too excited to do in the first place! Of course, now comes the semi-difficult part. I need to process the data, draw some conclusions, probably publish a few papers, and get a presentation made. Things need to happen quickly too, so while I’m done, I’m not. At least, not yet.

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The juggle

This wikihow image has me dying, this is exactly how I would attempt to juggle.

Every sunday without fail I make my list of tasks for the week ahead. Some of them are automated and repeat as needed, but most are specific to that week so I need to go in manually and add them. It’s nice to have everything written down in one place, in order, with times and reminders. I don’t have to stress out about remembering everything when I have a nice app on my phone that keeps me informed. Unfortunately there is a downside…

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Yet more experiments?!

Sticky notes are the best.

Another long day for me. Back to back experiments (with a short break in between thankfully). It’s a lot, but I’m hopeful that after it’s all done things will slow down at least for a moment, hint they won’t. In any case, I just need to make it to tomorrow then I get a break for a few days.

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A small reprieve

Well today my midday experiment was cancelled so now I have some time to relax. Just kidding, now I have time to work on the million other things I need to get done this week! That’s the problem with writing daily, a lot of it is the same stuff, but there are some minor differences with today’s list of things to do.

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A week of experiments

Don’t mind me, just trying to turn back time… lol

Most times work can be a trickle, we get one or two subjects a week and that’s all. 6-8 hours in the entire week dedicated to collecting data and the rest of the time I’m free to process data, work on other things I need to do, basically live day to day like I didn’t live in a lab. Most of the time. Sometimes it’s a flood, like this week and next, then I’m reminded that I definitely need to work.

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Catching up

This feels about right, I’ve been running on empty!

Sunday means getting ready for the rest of the week and what a week it will be. In my Co-PI’s lab we typically have a steady stream of people coming and going for experiments, this week (and probably next) it will be a flood. We’re trying to make my main-PI happy.

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Dominoes

It’s going to be a busy week for me. Seems to be a theme in my quest for that PhD to be honest. I’ve got data to process, papers to write, experiments to run, appointments to keep. It’s a lot of moving pieces that all need to be aligned at just the right moment. Dominoes, if I can get everything lined up nicely, the tasks will all fall just as easy.

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Experimental failure

Well that was a …flop. Today I had an experiment, had. There are a lot of moving parts when it comes to an experiment, especially in my line of research. Technology, can’t live with it, can’t live without it. Still, somedays I wish I was living in the future, one where I didn’t need to reset my router randomly. Then I would know for sure the singularity was close.

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Problem solved

Sometimes we don’t need to think outside the box, the box can stay closed for the moment…

Everyone go back to what you were doing, nothing to see here! Yesterday I had an existential crisis, one that could’ve derailed my technique and any hopes I had at introducing something new into the world. With it, my PhD dissertation topic, meaning I would be over two years in and I would need to reevaluate my goals. Let’s talk about what happened!

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A snag

No tears, please. It’s a waste of good suffering.

You know… that’s how it goes sometimes. You’re cruising along and everything looks great then, BAM! Totally derailed. What happened? Turns out I have a problem with my data. Not a problem persay, more like an anomaly, one that throws a rather large wrench into what WAS a perfectly running operation. Things were looking good, but now… I have a doubt.

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So much data

This has basically been my progression in this project.

Well I’ve processed (poorly) about half of my data. Now, when I say poorly I just mean the visuals for it are garbage and I need to tweek the sizes and things to make it look nicer, but the idea is that I’m more interested in finding something than I am in making it look pretty. I’ve processed two of four of my subjects and well I’m excited!

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The after

I’m trying to remain calm. I am an objective observer in the world of science and whatever the result, I will NOT let it cloud my emotions. I am neutral and I will remain that way… oh who am I kidding, I DID IT! Two years of planning and convincing people this would work. Then last night I had my first result, and it was a relief. It was a small step, but one I was afraid the data wouldn’t let me make.

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A busy day!

Yep I use this image a lot, but it’s such a great representation of our labs work!

Well I didn’t plan for it, but today is a busy day! I’ve got a lot going on at the moment so not a lot of time to write. Let’s just talk about what I’ve done and have left to do so I can get back to it.

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Process the data!

This is what raw EEG data looks like. Seriously, it’s a bunch of squiggles. However, we can make sense of it and we can even write some code to make sense of it in real-time so you can control things with your brain!!! <EVIL LAUGH>

It’s a process… that’s for sure. So you’re a scientist and you collect a ton of data, well now what? We’re talking about me of course. I did it, I collected a ton of data and now I need to do something useful with it. This is the part I wish I could skip over and get to the part where I get all the cool results, but I guess we’ve all got to start somewhere.

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Day 362: Experiment two

Just a giant case full of the equipment I borrowed from my university for experiments. Pretty sure the school’s office staff thing I was robbing the place though.

Well today is going to be a quick recap of my experiment yesterday. This will be just as much for me as it is for all of you. While I can’t go into the experiment details (since I’m working to publish this) I can give a pretty good overview like I did the last time around.

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Day 353: Experimental prep

Matlab

Sometimes a parfor loop in just doesn’t cut it because the things you want to do in that loop aren’t allowed (like save or load data a certain way). In those cases (see above) I can run multiple instances of MATLAB and process my data much more efficiently than if I did it one at a time. In this case I had 8 subjects that needed a rather long mathematical operation performed, so I did it all at the same time. It’s a little silly, but it worked! If you didn’t know you could do that, now you do!

Yesterday I told the story of how we got to this point, a long two year journey and next week I finally get to take the next step. It’s exciting, but it also means because of my flare-up, I’m behind on what I need to do to be ready. Not to worry though, I’m taking it slow so I don’t make whatever I have worse. What goes into an experiment like this? I’m glad you asked!

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Day 352: My experiment is coming!!

EEG Cap

I just really like how this photo came out. Here’s one of my lab mates gelling the electrodes for a pseudo-experiment. The scary looking syringe has a blunt tipped needle, we don’t break the skin in our lab so no pain, just a bit of gel to wash out of the hair once completed.

Okay I’ve got an experiment update today and I’m really excited to share because it feels real finally. I know I already wrote about some of this, but when I reread my previous post, it felt disjointed so let’s go over how we got here and what’s coming. I’m excited, are you excited? I’m excited!

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Day 350: In construction…

extruder

I redesigned my extruder to make it beefier, this has nothing to do with the post, but I’m really proud of it and wanted to share. Haha

Well today despite feeling like refried dog poop I have to go do experiments. It’s part of the job and to be honest I want to do it even though I feel like dried monkey vomit. I could go on, but you get the picture. I do have some more good news not related to yesterday’s news. So that is the conversation for the day.

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Day 343: On lab equipment

BrokenLaser1

Luckily this wasn’t my fault, but it does make for a good story now.

It was bound to happen eventually. We all knew it would, but we didn’t expect it to fail in such a spectacular manner. That is to say, all at once. One of the people I’m mentoring checked out some lab equipment since we had come up with a way to do experiments from the comfort (see: safety) of her home. That was the plan anyway…

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Day 295: Back in the lab

whole brain and spinal cord dissected

whole brain and spinal cord dissected

“What do you mean, I look nervous!?”

Well it finally happened. We’re doing experiments again. It’s kind of scary to be honest to be working in a hospital again when the pandemic is going on and we have protests still happening. While I could do without the pandemic, I hope with all my heart that the protests don’t end until the corrupt system that caused them ends first.

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Day 150: Surgery update

Well I’m alive, despite the VA’s best efforts. I’m struggling with some serious nausea to the point of vomiting, which has never happened to me before. I’m also in a lot of pain, but that was expected. In any case, start to finish (start as in the operating room and finish as in getting home so +30 minutes or so to the actual finish time) it took ~9 hours total good times for everyone. Anywho, I feel like death so I’ll write more later.


Day 149: An auspicious start

Brain

Well we did an experiment. I wish I could talk more about what we did, how we did, and why we did. Alas, I cannot. So instead, let’s talk about the vague how it went metric as in, maybe we found something maybe we didn’t, also this experiment highlights several quarks between the my school lab and the clinical lab.

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Day 148: Experimental design

 

EEGCap

Today is day one of ten for the time that I have to do some experiments. It’s an awkward time for sure, I mean surgery, school, etc. However, that’s just the way things work in academia, I actually had a break, so I’m ready to go to be honest. Which really means this isn’t horrible timing. I’ve already discussed the million things going on these weeks, but let’s talk about what goes into experiments, really.

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Day 143: Meeting with my two PI’s

Exo

Some of our exoskeletons from the lab

Today was an interesting set of events. I had my meeting with my two PI’s (which I still think would make a hilarious television show). The meeting went well, I’m very excited, but I’m also getting ready to be very, VERY busy. Let’s breakdown how it went shall we?

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Day 142: So you want to record from the brain…

EEG setup

My college helping me set up for the experiment I just did. This is how we add gel to the head, there is a tiny hole by each sensor, we then insert a blunt tip needle (we do NOT puncture the skin!) to add the gel between the sensor and the top of the head to eliminate the air gap caused by the hair. Unfortunately it looks scary, but we need something tiny to get around the sensor (if you look close you can seethe tiny, tiny opening each senor has). The lights on each sensor tell us how good of a connection we have, red means bad, yellow means we’re getting close, and green means good.

Today’s post was inspired by a conversation I was having yesterday in the comment section (you know who you are and thank you for the questions). I thought I would elaborate on how we record from the brain and why. There are a lot of different ways we can do this, some of them are super invasive and others are non-invasive. In the lab I work in now, we do things non-invasively there are good things about this and bad things about this, so let’s get into it!

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Day 132: Rat model validation!

stock photography Pet rat closeup

Yesterday I mentioned that I had some rat data to go through. It was an old(er) dataset, about five years-old to be exact, but it was one that was going to help me validate some of my findings. Unfortunately there existed no invasive human datasets to compare my human data to, so I needed to find an animal model, in this case a rat model. Let’s discuss the importance.

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Day 110: Experiment!

EEG setup

This is an actual photo from my experiment setup, one of my colleagues is adding gel to the sensors. While the giant syringe looks scary, it isn’t. It’s full of conductive gel that gets placed between the head and the sensor. There is no pain or puncturing of the skin involved. Afterwards the gel washes out of the hair. The real pain is sitting for ~30 minutes or so while we gel each electrode enough to get a good reading.

A few days ago I mentioned I did a thing, well an experimental thing really. It was… fun? It was definitely something. Overall it went well, but I said I would give everyone an update and I try to be a man of my word, so let’s do this.

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Day 106: Super Busy!!!!!

Today I had my experiment (yay), so now I need to process the data. I also sat in another PhD defense for one of our lab members, so now that I have a free second I wanted to give an update. Expect a longer post tomorrow, but for today, I have sooooo much work to do!

Until next time, don’t stop learning!


Day 105: Defense day #1

phd defense

This basically sums up today’s post…

Here we are another day another post. Today I will be spending the bulk of my time studying and getting my slides ready for the confrence I’ll be attending next week. That will be … fun? However today is also an important day for one of my fellow students, he’s defending his PhD.

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Day 104: Experiment Prep

EEG headset

It looks like things are moving a little quicker than I thought for me. As you may or may not know, I’m getting ready to do an experiment. Well, we finally (finally!) finalized the protocol and just in time too. While I won’t make the deadline for my project update, I will have some data to show when we get to the conference, which is a good consolation prize.

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Day 88: Experimental Headaches

EEG

EEG cap

I’ve talked about my impending deadlines a lot lately. I also mentioned that I had an experiment that I needed to do to meet a deadline, well it looks like we may or may not meet this goal. Let’s talk about the latest headaches.

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Day 20: Independent Events

correlation

By: xkcd

 

Because we introduced the central limit theorem last post, it’s time to introduce another important concept. The idea of independent events, while this may seem intuitive, it is one of the assumptions we make in parametric statistics, another concept we will define, but for now let’s jump into independence.*

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Day 19: The Central Limit theorem

 

 

CLT

Well here we are again, if you recall from our last post, we talked Bonferroni Correction. You may also recall that when the post concluded, there was no real topic for today. Well after some ruminating, before we jump into more statistics, we should talk about the central limit theorem. So let’s do a quick dive into what that is and why you should know it!*

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Day 18: The Bonferroni Correction

Keep calm and do the Bonferroni corrrection

Keep calm and do the Bonferroni corrrection

By now we are masters of statistics… right? Okay, not really, but we are getting there. So far we’ve covered two types of errors, type 1 which you can read about here, and type 2 which you can read about here. Armed with this new knowledge we can break into a way to correct for type 1 errors that come about from multiple comparisons. Sound confusing? Well, not for long, let’s break it down and talk Bonferroni.*

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Day 17: Type 2 errors

 

cat asleep

Last post we did a quick bit on type 1 errors. As with anything, there is more than one way to make an error. Today we are talking type 2 errors! They are related in the sense and we’ll go over what that means and compare the two right… now!*

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Day 16: Type 1 errors

tiny dog yawning

tiny dog yawning

We did it, we cracked the coin conundrum! We managed the money mystery! We checked the change charade! We … well you get the idea. Last post we (finally) determined if our coin was bias or not. Don’t worry, I won’t spoil it for you if you haven’t read it yet. I actually enjoyed working through a completely made up problem, so if you haven’t read it, you really should. Today we’re going to talk dogs, you’ll see what I mean, so let’s dive in.*

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Day 15: Significance, Part 3

Standard_deviation

Where does our observation fall on the probability density function?

It looks like we’ve arrived at part 3 of what is now officially a trilogy of posts on statistical significance. There is so much more to say I don’t want to quite call this the conclusion. Instead, let’s give a quick review of where we left off and we can get back to determining if an observed value is significant.*

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Day 14: Significance, Part 2

Bar graph showing z score across trials

Bar graph showing z score across trials

Z-score bar graph that I made just for all of you using some data I had laying around. If you’re new to statistics it may not make sense, but rest assured we will make sense of it all!

Well here we are two weeks into 365DoA, I was excited until I realized that puts us at 3.8356% of the way done. So if you remember from last post we’ve started our significance talk, as in what does it mean to have a value that is significant, what does that mean exactly, and how to do we find out? Today is the day I finally break, we’re going to have to do some math. Despite my best efforts I don’t think we can finish the significance discussion without it and still manage to make sense. With that, let’s just dive in.*

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Day 13: Significance, Part 1

Normally distributed data shown using a histogram plot

Normally distributed data shown using a histogram plot

Histogram of normally distributed data. It looks very… nomal. No it really is normally distributed, read on to find out what that means and how we can use it.

If you’ve read my last post I hinted that today we would discuss filtering. Instead I think I want to take this a different direction. That isn’t to say we won’t go over filtering, we most definitely will. Today I want to cover something else though, significance. So you’ve recorded your signal, took an ensemble average, and now how do we tell if it actually means something, or if you are looking at an artificial or arbitrary separation in your data (IE two separate conditions lead to no difference in your data). Let’s look at significance.*

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Day 12: Signal, cutting through the noise

example data

45 separate trials of very noisy data with the average of those trials (black). Believe it or not, this is actually very useful and very real data from something I am currently working on.

Noise, it can be troublesome. Whether you are studying and someone is being loud or you are trying to record something, noise is everywhere <stern look at people who talk during movies>. Interestingly enough the concept of noise in a signal recording sense isn’t all too different from dealing with talkative movie goers, so let’s talk noise!*

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